• Quick Neuron Mixed for 3D brain
  • Quick Neuron Mixed for 3D brain

Quick-Neuron™ Mixed

$79.00$299.00

Quick-Neuron™ Mixed allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within 10 days. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Mixed quickly produces neurons while remaining easy to use, convenient, and efficient. Differentiation into multi-types of neurons can be confirmed with anti-TUBB3 (tubulin-β3, a global marker for neurons) and anti-ChAT (choline acetyltransferase, a cholinergic neuron marker), anti-TH (Tyrosine hydroxylase, a dopaminergic neuron marker), anti-PV (parvalbumin, a GABAergic neuron marker), anti-vGLUT1 (vesicular Glutamate transporter 1, a glutamatergic neuron marker), anti-TPH2 (Tryptophan hydroxylase 2, a serotonergic neuron marker) antibodies. The each option contains enough reagents to use for 4 wells of a 24-well plate.

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SKU: EXGS-QNM-P Category: Tag:
You will get 15% volume discount by buying more than 3 kits!

Description

Quick-Neuron™ Mixed allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within 10 days for 3D brain etc. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Mixed quickly produces neurons while remaining easy to use, convenient, and efficient. Differentiation into multi-types of neurons can be confirmed with anti-TUBB3 (tubulin-β3, a global marker for neurons) and anti-ChAT (choline acetyltransferase, a cholinergic neuron marker), anti-TH (Tyrosine hydroxylase, a dopaminergic neuron marker), anti-PV (parvalbumin, a GABAergic neuron marker), anti-vGLUT1 (vesicular Glutamate transporter 1, a glutamatergic neuron marker), anti-TPH2 (Tryptophan hydroxylase 2, a serotonergic neuron marker) antibodies. Each option contains enough reagents to use for 4 wells of a 24-well plate.

Features

  • Rapid production: Stop waiting weeks. Quick-Neuron™ Mixed produces multi-types of neurons within 10 days.
  • Flexible choice: Complete kit includes all the necessary reagents such as media and well coating.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Mixed doesn’t.
  • Easy process: No more complex steps! Quick-Neuron™ Mixed has a simple and easy-to-understand protocol.

Protocol Outline

SeV Complete Kit

Applications

Stem Cell Culture, Differentiation and more.

Areas of Interest

3D tissues, 3D brain, 3D Bioprinting, Cell Replacement Therapy, Disease Modeling, Toxicity screening

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

Images


Representative phase contrast images of cultures treated with Quick-Neuron™ Mixed – SeV Complete Kit between Day 1 and Day 10 (objective 20x).

With Component K

Without Component K

Multiple neuronal markers to validate diverse subtypes of neuron in mixed neuronal differentiation. All antibodies were counterstained with Hoechst33324. Scale bars, 100 µm.

With Component K Without Component K

Gene expression profile of a culture of Quick-Neuron™ Mixed – SeV Complete Kit on Day 10

Specifications

Scale 4 wells of 24-well plate or 4-well plate
Contents SeV Complete Kit Five 500 µl tubes, two 1.5 ml tubes, and three 15 ml tubes
Maintenance Medium One 500 µl tube, and one 30 ml bottle
Shipping Conditions Dry ice
Storage Conditions -20°C or -80°C

Protocol/SDS

Protocol

SDS