• ipsc excitatory neurons merge

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CIRM Line ID: CW60231
Disease: Epilepsy
Gender: Male
Sampled age: 6
Ethnicity: Caucasian, not Latino

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SKU: EX-SeV-CW60231 Categories: , , Tag:

Description

Elixirgen Scientific’s proprietary transcription factor-based technology allows rapid and reproducible differentiation of human iPSCs into neurons without sacrificing the purity of the cells. Our Quick-Neuron™ Excitatory – Human iPSC-derived Neurons display typical neurite outgrowth and express a variety of neuronal markers, such as the pan-neuronal marker tubulin beta 3 class III (TUBB3) and the glutamatergic neuron marker vesicular glutamate transporter 1 (vGLUT1). When handled and maintained according to the instructions in this user guide, excitatory neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.

Protocol Outline

 

ipsc excitatory neurons protocol

Characterization

ipsc excitatory neurons
Quick-Neuron™ Excitatory cultures expressing the pan-neuronal marker TUBB3 and the glutamatergic neuron marker vGLUT1.

 

 

User Guides

SDS/Other

Donor data

Demographic (source: CIRM) Description EPILEPSY
Affected Status Yes
Product iPSC
Source PBMC
Sex Male
Age at Sampling 6 YR
Race Caucasian
Ethnicity Not Hispanic or Latino
Publications Cited 0
dbGap No
Biopsy Source Blood
Collection Neurodevelopmental Disorders
Remarks Height percentage < 5%
Cell Type Stem Cell
Tissue Type Induced pluripotent stem cell
Transformant Episomal
Species sapiens
Common Name human
Detailed Clinical Data false
Clinical data (source: CIRM) Age_at_first_seizure 7
Age_units Months
Age_at_last_seizure 6 years
Seizure_type Absence
Seizures_with_fever? Unknown
Seizures_without_fever? Yes
Being_treated_for_seizures_now? Yes
EEG_findings No data
  • Users do not need any additional license for the use of these differentiated cells even when cells are used for high throughput screening or drug discovery.
  • All donor consents include statements on the following:
    • Testing the cells’ DNA (this is referred to as the cell’s genetic code or sequence) and making the information known to other researchers
    • Changing some of the genetic code or sequence within these cells
    • Using cells to test or select drugs to treat disease
    • Transplanting cells or resulting products to humans or animals
      Distributing cells widely (nationally and internationally) for research, training or commercial medical product development
    • Future research and uses unforeseen at this time
  • Donors were screened and negative for HIV, HBV, and HCV. Donors under the age of 18 could opt out of HIV testing; all HCV-positive donors submitted skin punches, not peripheral blood, and were not screened for any infectious agents due to small volume of blood present in punches.
  • Human induced pluripotent stem cells were established by non-integrating episomal reprogramming. Reprogramming and quality control were performed by Cellular Dynamics International.

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Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.