• ipsc excitatory neurons merge

$1,499.00$6,499.00

CIRM Line ID: CW20026
Disease: Autism Spectrum Disorder
Gender: Male
Sampled age: 10
Ethnicity: Caucasian, Latino

Clear
SKU: EX-SeV-CW20026 Categories: , , Tag:

Description

Elixirgen Scientific’s proprietary transcription factor-based technology allows rapid and reproducible differentiation of human iPSCs into neurons without sacrificing the purity of the cells. Our Quick-Neuron™ Excitatory – Human iPSC-derived Neurons display typical neurite outgrowth and express a variety of neuronal markers, such as the pan-neuronal marker tubulin beta 3 class III (TUBB3) and the glutamatergic neuron marker vesicular glutamate transporter 1 (vGLUT1). When handled and maintained according to the instructions in this user guide, excitatory neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.

Protocol Outline

 

ipsc excitatory neurons protocol

Characterization

ipsc excitatory neurons
Quick-Neuron™ Excitatory cultures expressing the pan-neuronal marker TUBB3 and the glutamatergic neuron marker vGLUT1.

 

 

User Guides

SDS/Other

 

Donor data

Demographic (source: CIRM) Description AUTISM SPECTRUM DISORDER; ASD
Affected Status Yes
Product iPSC
Source PBMC
Sex Male
Age at Sampling 10 YR
Race Caucasian
Ethnicity Hispanic or Latino
Family CIRM00093
Relationship to Proband proband
Publications Cited 0
dbGap No
Diagnosis Age 2
Biopsy Source Blood
Collection Neurodevelopmental Disorders
Family Member Number 1
Cell Type Stem Cell
Tissue Type Induced pluripotent stem cell
Transformant Episomal
Species sapiens
Common Name human
Subject Type concordant sib pair
Detailed Clinical Data false
Clinical data (source: CIRM) ASD ASD
Number_of_siblings_with_ASD 1
Symptoms_Present_in_the_First_Year_of_Life None
ADOS_Test_Used ADOS 2
Communication_and_Social 19
Stereotyped_Behaviors_and_Restricted_Interests 3
Full_Scale_IQ 52
Verbal_IQ 46
Non_Verbal_IQ 61
Test_used Stanford Binet
ASDMedicalFamilyHistory ASDMedicalFamilyHistory
Diagnosis Autism Spectrum Disorder
Birthweight_(kgs) 2.35
Birth_order 4
Number_of_siblings_(includes_half_and_whole_siblin 3
Weight_% 3
Height_% 45.1
Head_Circumference_(cm) 54.25
Maternal_age_(years) 35
Maternal_age_at_delivery_(years) 25
Paternal_age_(years) 50
Paternal_age_at_delivery_(years) 40
Chromosome_test_results Not Available
Consanguinity No

 

  • Users do not need any additional license for the use of these differentiated cells even when cells are used for high throughput screening or drug discovery.
  • All donor consents include statements on the following:
    • Testing the cells’ DNA (this is referred to as the cell’s genetic code or sequence) and making the information known to other researchers
    • Changing some of the genetic code or sequence within these cells
    • Using cells to test or select drugs to treat disease
    • Transplanting cells or resulting products to humans or animals
      Distributing cells widely (nationally and internationally) for research, training or commercial medical product development
    • Future research and uses unforeseen at this time
  • Donors were screened and negative for HIV, HBV, and HCV. Donors under the age of 18 could opt out of HIV testing; all HCV-positive donors submitted skin punches, not peripheral blood, and were not screened for any infectious agents due to small volume of blood present in punches.
  • Human induced pluripotent stem cells were established by non-integrating episomal reprogramming. Reprogramming and quality control were performed by Cellular Dynamics International.

Shipping

Products will be shipped through FedEx Corporation’s services, and are available for purchase internationally. Shipping cost will be estimated, calculated, and displayed along with your order total in the checkout screen.

Payment

Payment will be processed by the Elixirgen Scientific store page’s Paypal plugin. The Paypal plugin offers payment through multiple credit and banking services, as well as Paypal itself. If there are any questions regarding alternative payment methods or bulk purchases, please email orders@elixirgenscientific.com.

Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.