• Cholinergic neuron

$349.00

Quick-Neuron™ Cholinergic allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within one week. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Cholinergic quickly produces neurons while remaining easy to use, convenient, and efficient. The each option contains enough reagents to use for 4 wells of a 24-well plate.

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SKU: EXGS-QNC-P Category:
You will get 15% volume discount by buying more than 3 kits!

Description

Quick-Neuron™ Cholinergic allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within one week. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Cholinergic quickly produces neurons while remaining easy to use, convenient, and efficient. The each option contains enough reagents to use for 4 wells of a 24-well plate.

Features

  • Rapid production: Stop waiting weeks. Quick-Neuron™ Cholinergic produces cholinergic neurons in under one week.
  • Flexible choice: Complete kit includes all the necessary reagents such as media and well coating.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Cholinergic doesn’t.
  • Easy process: No more complex steps! Quick-Neuron™ Cholinergic has a simple and easy-to-understand protocol.

Protocol Outline

SeV Complete Kit

mRNA Complete Kit

Applications

Stem Cell Culture, Differentiation and more.

Areas of Interest

Diseases Related to the Brain, Spine, and Nerves such as Amyotrophic lateral sclerosis (ALS), multifocal motor neuropathy, primary lateral sclerosis, progressive muscular atrophy, progressive bulbar palsy, or pseudobulbar palsy

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

FAQ

Visit our FAQ page for this product.

Images


Movie depicting the calcium transients of differentiated neurons on Day 7. After loading with Fluo-4, cells were stimulated with 40 Hz pulse.
[Published originally as Supplementary Movie M1 in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]


Repeated multiple action potentials induced by current injections in the current clamp mode (Day 10).
[Published originally as Figure 5g in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]


Immuno-staining images of a culture of Quick-Neuron™ Cholinergic – SeV Complete Kit on Day 5 (objective 20x).


Immuno-staining images of a culture of Quick-Neuron™ Cholinergic – mRNA Complete Kit on Day 7.


Gene expression profile of a culture of Quick-Neuron™ Cholinergic – SeV Complete Kit on Day 5.


Gene expression profile of a culture of Quick-Neuron™ Cholinergic – mRNA Complete Kit on Day 7.


Representative phase contrast images of cultures treated with Quick-Neuron™ Cholinergic – SeV Complete Kit (objective 10x).


Representative phase contrast images of cultures on wells treated with Quick-Neuron™ Cholinergic – SeV Complete Kit (objective 20x).


Representative phase contrast images of cultures on glass cover-slips treated with Quick-Neuron™ Cholinergic – SeV Complete Kit.


Representative phase contrast images of cultures treated with Quick-Neuron™ Cholinergic – mRNA Complete Kit (objective 10x).


Representative phase contrast images of cultures treated with Quick-Neuron™ Cholinergic – mRNA Complete Kit (objective 20x).

Specifications

SeV Complete Kit Scale 4 wells of a 24-well plate or 4-well plate
Contents Five 500 µl tubes, two 15 ml tubes, and two 2.0 ml tubes
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See product protocol)
SeV Complete Kit for 96-well micro plate format Scale 48 wells of a 96-well microplate
Contents Six 500 µl tubes, four 15 ml tubes, and one 30 ml bottle
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See product protocol)
mRNA Complete Kit Scale 4 wells of a 24-well plate or 4-well plate
Contents Eight 500 µl tubes, three 15 ml tubes, and two 2.0 ml tubes
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See product protocol)
Maintenance Medium Scale 4 wells of a 24-well or 4-well plate for 2 weeks
Contents One 500 µl tube and one 30 ml bottle
Shipping Conditions Dry ice
Storage Conditions -20°C

Protocol/SDS

Protocol

SDS