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Quick-Neuron™ Cholinergic

$69.00$299.00

Quick-Neuron™ Cholinergic allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within one week. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Cholinergic quickly produces neurons while remaining easy to use, convenient, and efficient. The each option contains enough reagents to use for 4 wells of a 24-well plate.

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SKU: EXGS-QNC-P Category:
You will get 15% volume discount by buying more than 3 kits!

Description

Quick-Neuron™ Cholinergic allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within one week. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Cholinergic quickly produces neurons while remaining easy to use, convenient, and efficient. The each option contains enough reagents to use for 4 wells of a 24-well plate.

Features

  • Rapid production: Stop waiting weeks. Quick-Neuron™ Cholinergic produces cholinergic neurons in under one week.
  • Flexible choice: Complete kit includes all the necessary reagents such as media and well coating. Booster transcription factors can be added to user’s existing protocol.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Cholinergic doesn’t.
  • Easy process: No more complex steps! Quick-Neuron™ Cholinergic has a simple and easy-to-understand protocol.

Protocol Outline

SeV Complete Kit

mRNA Complete Kit

Applications

Stem Cell Culture, Differentiation and more.

Areas of Interest

Diseases Related to the Brain, Spine, and Nerves such as Amyotrophic lateral sclerosis (ALS), multifocal motor neuropathy, primary lateral sclerosis, progressive muscular atrophy, progressive bulbar palsy, or pseudobulbar palsy

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

Images


Movie depicting the calcium transients of differentiated neurons on Day 7. After loading with Fluo-4, cells were stimulated with 40 Hz pulse.
[Published originally as Supplementary Movie M1 in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]


Repeated multiple action potentials induced by current injections in the current clamp mode (Day 10).
[Published originally as Figure 5g in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]


Immunostaining images of a culture of Quick-Neuron™ Cholinergic – SeV Complete Kit on Day 5 (objective 20x).


Immunostaining images of a culture of Quick-Neuron™ Cholinergic – mRNA Complete Kit on Day 7 (objective 20x).


Gene expression profile of a culture of Quick-Neuron™ Cholinergic – SeV Complete Kit on Day 8


Gene expression profile of a culture of Quick-Neuron™ Cholinergic – mRNA Complete Kit on Day 8

Specifications

Scale 4 wells of 24-well plate or 4-well plate
Contents SeV Complete Kit Eight 500 µl tubes, two 2 ml tubes, three 15 ml tubes
SeV Booster Transcription Factor One 500 µl tube
mRNA Complete Kit Eight 500 µl tubes, two 2 ml tubes, three 15 ml tubes
mRNA Booster Transcription Factor Four 500 µl tubes
Maintenance Medium Two 15 ml tubes
Shipping Conditions Dry ice
Storage Conditions -20°C

Protocol/SDS

Protocol

SDS

Shipping/Legal/Payment