• Quick-miniBrain

$999.00

Quick-miniBrain™ allows differentiated neurons and endothelial cells to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within 14 days for 3D brain etc. Unlike other products on the market that take nearly a month or longer to generate results, Quick-miniBrain™ quickly produces neurons while remaining easy to use, convenient, and efficient. Differentiation into multi-types of neurons and endothelial cells can be confirmed with anti-TUBB3 (tubulin-β3, a global marker for neurons), anti-ChAT (choline acetyltransferase, a cholinergic neuron marker), anti-TH (Tyrosine hydroxylase, a dopaminergic neuron marker), anti-PV (parvalbumin, a GABAergic neuron marker), anti-vGLUT1 (vesicular Glutamate transporter 1, a glutamatergic neuron marker), anti-TPH2 (Tryptophan hydroxylase 2, a serotonergic neuron marker), and anti-CD31 (endothelial cell marker) antibodies. Each option contains enough reagents to complete differentiation.

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SKU: EXGS-QMB-P Categories: , Tag:
You will get 15% volume discount by buying more than 3 kits!

Description

US

Quick-miniBrain™ allows differentiated neurons and endothelial cells to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within 14 days for 3D brain etc. Unlike other products on the market that take nearly a month or longer to generate results, Quick-miniBrain™ quickly produces neurons while remaining easy to use, convenient, and efficient. Differentiation into multi-types of neurons and endothelial cells can be confirmed with anti-TUBB3 (tubulin-β3, a global marker for neurons), anti-ChAT (choline acetyltransferase, a cholinergic neuron marker), anti-TH (Tyrosine hydroxylase, a dopaminergic neuron marker), anti-PV (parvalbumin, a GABAergic neuron marker), anti-vGLUT1 (vesicular Glutamate transporter 1, a glutamatergic neuron marker), anti-TPH2 (Tryptophan hydroxylase 2, a serotonergic neuron marker), and anti-CD31 (endothelial cell marker) antibodies. Each option contains enough reagents to complete differentiation.

Features

  • Rapid production: Stop waiting months. Quick-miniBrain™ produces multi-types of neurons and endothelial cells within 14 days.
  • Flexible choice: Complete kit includes all the necessary reagents such as media and well coating.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-miniBrain™ doesn’t.
  • Easy process: No more complex steps! Quick-miniBrain™ has a simple and easy-to-understand protocol.

Protocol Outline

SeV neuron and mRNA EC Kit

Applications

Stem Cell Culture, Differentiation and more.

Areas of Interest

3D tissues, 3D brain, 3D Bioprinting, Cell Replacement Therapy, Disease Modeling, Toxicity screening

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

FAQ

Visit our FAQ page for this product.

Images


Immuno-staining images of cultures treated by Quick-miniBrain™ – SeV neuron and mRNA EC Kit on Day 28 with Component K. Scale bars, 100 µm.


Immuno-staining images of cultures treated by Quick-miniBrain™ – SeV neuron and mRNA EC Kit on Day 28 without Component K. Scale bars, 100 µm.


Gene expression profile of a culture of Quick-miniBrain™ – SeV neuron and mRNA EC Kit on Day 28 with Component K.

Specifications

SeV neuron and mRNA EC Kit Scale 4 wells of a 24-well plate or 4-well plate
Contents Eight 500 µl tubes, two 2.0 ml tubes, three 15 ml tubes, and two 60 ml bottles
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See product protocol)
Maintenance Medium Scale 4 wells of a 24-well or 4-well plate for 2 weeks
Contents Two 500 µl tubes and one 30 ml bottle
Shipping Conditions Dry ice
Storage Conditions -20°C

Protocol/SDS

Protocol

SDS