$99.00

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Mesendoderm Cells
Scale: 8 wells of 24-well plate

You will get 15% volume discount by buying more than 3 kits!

Description

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Mesendoderm Cells
Scale: 8 wells of 24-well plate

Quick-Tissue™ Mesendoderm Booster accelerates human pluripotent stem cell (hPSC) differentiation into tissues from the Mesendoderm layer by strongly suppressing the pluripotency maintenance program in hPSCs. This product works for both human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Once this product is added to your hPSC differentiation method of choice, you will get mature cells in a much shorter time period. The Mesendoderm Booster is already used in Quick-Muscle™ Skeletal and upcoming Quick-Tissue™ Differentiation Kits of Mesendoderm-derived tissues such as blood and vascular endothelium.
This product is intended to be used at the beginning of hPSC differentiation. The booster should be added immediately after the PSCs are put into differentiation conditions. However, users may want to add the booster at alternative timings before or after the culture conditions of PSC are switched from the pluripotency maintenance to differentiation to further optimize their protocols.

Features

  • Boost your cell differentiation speed
  • Flexible: Use the Mesendoderm Booster with your existing differentiation protocol.
  • Works with most major differentiation methods such as transient transfection, gene, and medium-based induction

Image

Representative immunofluorescence images of human iPSC cultures maintained under skeletal muscle differentiation conditions with (right) or without (left) Mesendoderm Booster. These cultures were fixed with 4% paraformaldehyde 7 days after the initiation of differentiation. Blue fluorescence shows nuclei stained with Hoechst 33258 and red fluorescence indicates the presence of myosin heavy chain. Bars, 50 μm.

Applications

Stem Cell Culture, Differentiation and more.

Reference

Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko. “Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells.” Development 2016. 143: 3674-3685; doi: 10.1242/dev.139360
Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH (2018). “Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.” Sci Rep. 2018 8(1):1189.

Specifications

Scale 2 wells of a 24-well or 4-well plate x 3
Contents Three tubes (55 µl x 3)
Shipping Conditions Dry ice
Storage Conditions -80°C

FAQ

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Protocol/SDS

Protocol

SDS

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