Product Name: Quick-Neuron™ Mixed – Human iPSC-derived Neurons (F, 21 yr) – Healthy Control
Quantity: ~1M viable cells
CIRM Line ID: CW70067
Disease: Healthy Donor
Sampled age: 21
Elixirgen Scientific’s proprietary transcription factor-based technology allows rapid and reproducible differentiation of human iPSCs into neurons without sacrificing the purity of the cells. Our Quick-Neuron™ Mixed – Human iPSC-derived Neurons exhibit typical neuronal morphology with outgrowing neurites and express markers characteristic of a variety of neuronal subtypes, including the pan-neuronal marker tubulin beta 3 class III (TUBB3), the glutamatergic neuron marker vesicular glutamate transporter 1 (vGLUT1), the cholinergic neuron marker choline acetyltransferase (ChAT), the dopaminergic neuron marker tyrosine hydroxylase (TH), the serotonergic neuron marker tryptophan hydroxylase 2 (TPH2), and the GABAergic neuron marker glutamate decarboxylase 1, 67 kDa isoform (GAD1/GAD67). When thawed and maintained according to the instructions in the User Guide, the iPSC-derived neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.
- Functionally validated
- Highly pure population
- No genetic footprint
From Day 10, users may maintain differentiated neurons in the maintenance medium best suited for their needs, though we recommend Quick-Neuron™ Mixed – Maintenance Medium.
Quick-Neuron™ Mixed cultures expressing the pan-neuronal marker TUBB3 and glutamatergic neuron marker vGLUT1 on Day 7 post-thaw.
Quick-Neuron™ Mixed cultures on Days 1 through 10 post-thaw (4x and 10x objective).
- Quick-Neuron™ Mixed – Human iPSC-derived Neurons
- Quick-Neuron™ Mixed – Maintenance Medium (sold separately here)
|Demographic (source: CIRM)||Description||CONTROL: NO COGNITIVE DECLINE (SELF-REPORTED) | CONTROL: NON-DIABETIC WITHOUT AMD/DR/POAG (BASED ON EYE EXAM) | CONTROL: NO CHRONIC LUNG DISEASE (SELF REPORTED)|
|Age at Sampling||21 YR|
|Ethnicity||Not Hispanic or Latino|
|Collection||Alzheimer’s Disease,Blinding Eye Diseases,Control,Lung Diseases|
|Cell Type||Stem Cell|
|Tissue Type||Induced pluripotent stem cell|
|Detailed Clinical Data||false|
|Clinical data (source: CIRM)||CognitiveSharedControl||CognitiveSharedControl|
- Frozen cells SDS
- Component N SDS
- License Statement
- CIRM iPS cell line Certificate of Analysis for CW70067
Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma,Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. ” Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367
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Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.