Quick-Neuron™ Mixed – Human iPSC-derived Neurons (F, 21 yr) – Healthy Control

Description

Elixirgen Scientific’s proprietary transcription factor-based technology allows rapid and reproducible differentiation of human iPSCs into neurons without sacrificing the purity of the cells. Our Quick-Neuron™ Mixed – Human iPSC-derived Neurons exhibit typical neuronal morphology with outgrowing neurites and express markers characteristic of a variety of neuronal subtypes, including the pan-neuronal marker tubulin beta 3 class III (TUBB3), the glutamatergic neuron marker vesicular glutamate transporter 1 (vGLUT1), the cholinergic neuron marker choline acetyltransferase (ChAT), the dopaminergic neuron marker tyrosine hydroxylase (TH), the serotonergic neuron marker tryptophan hydroxylase 2 (TPH2), and the GABAergic neuron marker glutamate decarboxylase 1, 67 kDa isoform (GAD1/GAD67). When thawed and maintained according to the instructions in the User Guide, the iPSC-derived neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.

Features

• Functionally validated 
• Highly pure population  
• No genetic footprint 

Protocol Outline

From Day 10, users may maintain differentiated neurons in the maintenance medium best suited for their needs, though we recommend Quick-Neuron™ Mixed – Maintenance Medium.

Characterization

Quick-Neuron™ Mixed cultures expressing the pan-neuronal marker TUBB3 and glutamatergic neuron marker vGLUT1 on Day 7 post-thaw.

Quick-Neuron™ Mixed cultures on Days 1 through 10 post-thaw (4x and 10x objective).

User Guides

• Quick-Neuron™ Mixed – Human iPSC-derived Neurons
• Quick-Neuron™ Mixed – Maintenance Medium (sold separately here)

Donor Information

Demographic (source: CIRM)	Description	CONTROL: NO COGNITIVE DECLINE (SELF-REPORTED) | CONTROL: NON-DIABETIC WITHOUT AMD/DR/POAG (BASED ON EYE EXAM) | CONTROL: NO CHRONIC LUNG DISEASE (SELF REPORTED)
	Affected Status	No
	Product	iPSC
	Source	PBMC
	Sex	Female
	Age at Sampling	21 YR
	Race	Asian
	Ethnicity	Not Hispanic or Latino
	Publications Cited	0
	dbGap	No
	Biopsy Source	Blood
	Collection	Alzheimer’s Disease,Blinding Eye Diseases,Control,Lung Diseases
	Cell Type	Stem Cell
	Tissue Type	Induced pluripotent stem cell
	Transformant	Episomal
	Species	sapiens
	Common Name	human
	Detailed Clinical Data	false
Clinical data (source: CIRM)	CognitiveSharedControl	CognitiveSharedControl
	History_of_significant_memory_changes_in_the_past_	No
	History_of_major_depression_or_psychiatric_illness	No
	History_of_seizures_alcohol_or_drug_abuse	No
	Montreal_Cognitive_Assessment_(MOCA)_score_>_25/30	Don’t know
	Parent_or_sibling_with_Alzheimers_Disease_with_ons	No
	OphthalmicSharedControl	OphthalmicSharedControl
	Glaucoma	No
	Age-Related_Macular_Degeneration	No
	Diabetic	No
	Diabetic_retinopathy	No
	Family_history_of_eye_diseases	No
	PulmonarySharedControl	PulmonarySharedControl
	Smoking_Status	never
	History_of_chronic_lung_disease	No
	Experienced_a_cough_at_least_4_days_a_week_for_at_	No
	Experienced_breathlessness_that_limits_ability_to_	None
	Used_or_been_prescribed_oxygen	No
	Had_a_transplant_(bone_marrow_peripheral_stem_cell	No
	Active_cancer_or_cancer_diagnosis_in_the_past_2_ye	No
	Family_history_of_pulmonary_fibrosis	No

SDS/Other

• Frozen cells SDS
• Component N SDS
• License Statement
• CIRM iPS cell line Certificate of Analysis for CW70067

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma,Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. ” Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

Shipping

Products will be shipped through FedEx Corporation’s services, and are available for purchase internationally. Shipping cost will be estimated, calculated, and displayed along with your order total in the checkout screen.

Payment

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Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.