$1,499.00

Product Name: Quick-Neuron™ Mixed – Human iPSC-derived Neurons (F, 18 yr) – Autism Spectrum Disorder
Quantity: ~1M viable cells
CIRM Line ID: CW20090
Disease: Autism Spectrum Disorder
Gender: Female
Sampled age: 18
Ethnicity: Asian

SKU: MN-SeV-CW20090 Categories: , ,

Description

Elixirgen Scientific’s proprietary transcription factor-based technology allows rapid and reproducible differentiation of human iPSCs into neurons without sacrificing the purity of the cells. Our Quick-Neuron™ Mixed – Human iPSC-derived Neurons exhibit typical neuronal morphology with outgrowing neurites and express markers characteristic of a variety of neuronal subtypes, including the pan-neuronal marker tubulin beta 3 class III (TUBB3), the glutamatergic neuron marker vesicular glutamate transporter 1 (vGLUT1), the cholinergic neuron marker choline acetyltransferase (ChAT), the dopaminergic neuron marker tyrosine hydroxylase (TH), the serotonergic neuron marker tryptophan hydroxylase 2 (TPH2), and the GABAergic neuron marker glutamate decarboxylase 1, 67 kDa isoform (GAD1/GAD67). When thawed and maintained according to the instructions in the User Guide, the iPSC-derived neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.

Features

  • Functionally validated 
  • Highly pure population  
  • No genetic footprint 

Protocol Outline

ipsc neuron workflow

From Day 10, users may maintain differentiated neurons in the maintenance medium best suited for their needs, though we recommend Quick-Neuron™ Mixed – Maintenance Medium.

Characterization

ipsc neurons

Quick-Neuron™ Mixed cultures expressing the pan-neuronal marker TUBB3 and glutamatergic neuron marker vGLUT1 on Day 7 post-thaw.

ipsc neurons bf

Quick-Neuron™ Mixed cultures on Days 1 through 10 post-thaw (4x and 10x objective).

User Guides

Donor Information

Demographic (source: CIRM) Description AUTISM SPECTRUM DISORDER; ASD
Affected Status Yes
Product iPSC
Source PBMC
Sex Female
Age at Sampling 18 YR
Race Asian
Ethnicity Not Hispanic or Latino
Publications Cited 0
dbGap No
Diagnosis Age 3
Biopsy Source Blood
Collection Neurodevelopmental Disorders
Cell Type Stem Cell
Tissue Type Induced pluripotent stem cell
Transformant Episomal
Species sapiens
Common Name human
Detailed Clinical Data false
Clinical data (source: CIRM) ASD ASD
Number_of_siblings_with_ASD 0
Symptoms_Present_in_the_First_Year_of_Life No data
Communication_-_Verbal 20
Communication_-_Nonverbal 12
Restricted_Repetitive_and_Stereotyped_Patterns_of_ 6
Abnormality_of_Development_Evident_at_or_Before_36 4
Qualitative_Impairment_in_Reciprocal_Social_Intera 29
ADOS_Test_Used ADOS 2
Communication_and_Social 13
Stereotyped_Behaviors_and_Restricted_Interests 5
Full_Scale_IQ 40
Verbal_IQ 43
Non_Verbal_IQ 40
Test_used Stanford Binet
ASDMedicalFamilyHistory ASDMedicalFamilyHistory
Diagnosis Autism Spectrum Disorder
Birthweight_(kgs) 3.88
Birth_order 2
Number_of_siblings_(includes_half_and_whole_siblin 1
Weight_% 50
Height_% 55.88
Head_Circumference_(cm) 59
Maternal_age_(years) 54
Maternal_age_at_delivery_(years) 36
Paternal_age_(years) 59
Paternal_age_at_delivery_(years) 41
Chromosome_test_results Not Available
Consanguinity No

SDS/Other

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma,Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. ” Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

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Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.