$1,499.00

Product Name: Quick-Neuron™ Mixed – Human iPSC-derived Neurons (M, 36 yr) – Fatty Liver Disease
Quantity: ~1M viable cells
CIRM Line ID: CW10130
Disease: Fatty Liver Disease
Gender: Male
Sampled age: 36
Ethnicity: Asian

SKU: MN-SeV-CW10130 Categories: , ,

Description

Elixirgen Scientific’s proprietary transcription factor-based technology allows rapid and reproducible differentiation of human iPSCs into neurons without sacrificing the purity of the cells. Our Quick-Neuron™ Mixed – Human iPSC-derived Neurons exhibit typical neuronal morphology with outgrowing neurites and express markers characteristic of a variety of neuronal subtypes, including the pan-neuronal marker tubulin beta 3 class III (TUBB3), the glutamatergic neuron marker vesicular glutamate transporter 1 (vGLUT1), the cholinergic neuron marker choline acetyltransferase (ChAT), the dopaminergic neuron marker tyrosine hydroxylase (TH), the serotonergic neuron marker tryptophan hydroxylase 2 (TPH2), and the GABAergic neuron marker glutamate decarboxylase 1, 67 kDa isoform (GAD1/GAD67). When thawed and maintained according to the instructions in the User Guide, the iPSC-derived neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.

Features

  • Functionally validated 
  • Highly pure population  
  • No genetic footprint 

Protocol Outline

ipsc neuron workflow

From Day 10, users may maintain differentiated neurons in the maintenance medium best suited for their needs, though we recommend Quick-Neuron™ Mixed – Maintenance Medium.

Characterization

ipsc neurons

Quick-Neuron™ Mixed cultures expressing the pan-neuronal marker TUBB3 and glutamatergic neuron marker vGLUT1 on Day 7 post-thaw.

ipsc neurons bf

Quick-Neuron™ Mixed cultures on Days 1 through 10 post-thaw (4x and 10x objective).

User Guides

Donor Information

Demographic (source: CIRM) Description FATTY LIVER DISEASE | DEFINITE NASH
Affected Status Yes
Product iPSC
Source PBMC
Sex Male
Age at Sampling 36 YR
Race Asian
Ethnicity Not Hispanic or Latino
Publications Cited 0
dbGap No
Biopsy Source Blood
Collection Liver Diseases
Cell Type Stem Cell
Tissue Type Induced pluripotent stem cell
Transformant Episomal
Species sapiens
Common Name human
Detailed Clinical Data false
Clinical data (source: CIRM) LiverDisordersNASH LiverDisordersNASH
Diagnosis Fatty Liver Disease
Body_Mass_Index 28.5
Hypertension No
Diabetes No
Hyperlipidemia Yes
Alcohol_Consumption None
HBsAg_negative Yes
HCV_antibody_negative Yes
ANA_or_IgG_result normal
Liver_biopsy_(year) 2014
Steatosis_score_(0-3) 2
Ballooning_score_(0-2) 1
Lobular_inflammation_score_(0-3) 1
NAS_score_(0-8) 4
Fibrosis_score_(0-4) 0
NASH_diagnosis_by_pathologist definite NASH

SDS/Other

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma,Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. ” Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

Shipping

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Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.