$299.00$999.00

Product Name: Quick-Neuron™ Mixed – SeV Kit
Category: Human iPS/ES cell differentiation reagent
Tissue Type: Mixed Neurons
Scale: 4 wells of a 24-well plate (Small) or 6 wells of a 6-well plate/100-mm dish (Large)

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Description

The Quick-Neuron™ Mixed SeV Kit allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within 10 days. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Mixed quickly produces neurons while remaining easy to use, convenient, and efficient. Differentiation into multi-types of neurons can be confirmed with anti-TUBB3 (tubulin-β3, a global marker for neurons), anti-vGLUT1 (vesicular Glutamate transporter 1, a glutamatergic neuron marker), anti-ChAT (choline acetyltransferase, a cholinergic neuron marker), anti-TH (Tyrosine hydroxylase, a dopaminergic neuron marker), anti-GAD67 (glutamate decarboxylase 1, 67 kDa isoform (GAD1/GAD67, a GABAergic neuron marker), anti-TPH2 (Tryptophan hydroxylase 2, a serotonergic neuron marker) antibodies.

Features

  • Rapid production: Stop waiting weeks. Quick-Neuron™ Mixed – SeV Kit produces multiple subtypes of functional neurons within 10 days.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Mixed – SeV Kit doesn’t.
  • Easy process: No more complex steps! Quick-Neuron™ Mixed – SeV Kit has a simple and easy-to-understand protocol.

Protocol Outline

ips differentiation protocol

From Day 10, users may maintain differentiated neurons in the maintenance medium best suited for their needs, though we recommend Quick-Neuron™ Mixed – Maintenance Medium.

Characterization

ipsc neurons bf

 Representative images of cultures by Quick-Neuron™ Mixed cultures on Days 1 through 10 (4x and 10x objective).

ipsc neurons

Quick-Neuron™ Mixed cultures expressing the pan-neuronal marker TUBB3 and glutamatergic neuron marker vGLUT1.

Specifications

Scale 4 wells of a 24-well plate (small) or 6 wells of a 6-well plate/100-mm dish (large)
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See User Guides for more information).

User Guides

SDS/Other

FAQ

Visit our FAQ page for this product.

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

Shipping

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Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.