$299.00$999.00

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Cholinergic Neurons
Scale: 4 wells of a 24-well plate or 6 wells of a 6-well plate/a 100 mm dish

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Description

The Quick-Neuron™ Cholinergic – SeV Kit facilitates rapid and efficient differentiation of human iPS or ES cells into a population of primarily cholinergic neurons in just 6 days. Our proprietary transcription factor-based stem cell differentiation method uses Sendai virus to produce highly pure populations of neurons without a genetic footprint. Quick-Neuron™ Cholinergic differentiated cell cultures display typical neurite outgrowth and express a variety of neuronal markers, such as the pan-neuronal marker tubulin beta 3 class III (TUBB3) and the cholinergic marker choline acetyl-transferase (ChAT). When handled and maintained according to the instructions in this user guide, cholinergic neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.

Features

  • Rapid production: Stop waiting weeks. Quick-Neuron™ Cholinergic produces cholinergic neurons in under one week.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Cholinergic doesn’t.
  • Easy process: No more complex steps! Quick-Neuron™ Cholinergic has a simple and easy-to-understand protocol.

Protocol Outline

Quick-Neuron™ Cholinergic – SeV Kit (Small)

 

ipsc cholinergic neurons protocol

From Day 6, users may maintain differentiated neurons in the maintenance medium best suited for their needs, though we recommend Quick-Neuron™ Cholinergic – Maintenance Medium.

 

Quick-Neuron™ Cholinergic – SeV Kit (Large)

 

ipsc cholinergic neurons

From Day 6, users may maintain differentiated neurons in the maintenance medium best suited for their needs, though we recommend Quick-Neuron™ Cholinergic – Maintenance Medium.

Characterization

ipsc cholinergic neurons
Quick-Neuron™ Cholinergic cultures expressing the pan-neuronal marker TUBB3 and the cholinergic neuron marker ChAT at day 6.

Specifications

Scale 4 wells of a 24-well plate (small) or 6 wells of a 6-well plate/100-mm dish (large)
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See User Guides for more information)

User Guides

SDS/Other

FAQ

Visit our FAQ page for this product.

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

Shipping

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Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.