• Cholinergic neuron

$349.00

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Cholinergic Neurons
Scale: 4 wells of 24-well plate

Description

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Cholinergic Neurons
Scale: 4 wells of 24-well plate

Quick-Neuron™ Cholinergic allows differentiated neurons to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within one week. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Neuron™ Cholinergic quickly produces neurons while remaining easy to use, convenient, and efficient. The each option contains enough reagents to use for 4 wells of a 24-well plate.

Features

  • Rapid production: Stop waiting weeks. Quick-Neuron™ Cholinergic produces cholinergic neurons in under one week.
  • Flexible choice: Complete kit includes all the necessary reagents such as media and well coating.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Cholinergic doesn’t.
  • Easy process: No more complex steps! Quick-Neuron™ Cholinergic has a simple and easy-to-understand protocol.

Protocol Outline

Applications

Stem Cell Culture, Differentiation and more.

Areas of Interest

Diseases Related to the Brain, Spine, and Nerves such as Amyotrophic lateral sclerosis (ALS), multifocal motor neuropathy, primary lateral sclerosis, progressive muscular atrophy, progressive bulbar palsy, or pseudobulbar palsy

Specifications

Scale 4 wells of a 24-well plate
Contents Four 500 µl tubes and three 2.0 ml tubes
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See product protocol)

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

FAQ

Visit our FAQ page for this product.

Images


Movie depicting the calcium transients of differentiated neurons on Day 7. After loading with Fluo-4, cells were stimulated with 40 Hz pulse.
[Published originally as Supplementary Movie M1 in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]

Repeated multiple action potentials induced by current injections in the current clamp mode (Day 10).
[Published originally as Figure 5g in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]

Immuno-staining images of a culture of Quick-Neuron™ Cholinergic – SeV Complete Kit on Day 5 (objective 20x).

Gene expression profile of a culture of Quick-Neuron™ Cholinergic – SeV Complete Kit on Day 5.

Representative phase contrast images of cultures treated with Quick-Neuron™ Cholinergic – SeV Complete Kit (objective 10x).

Representative phase contrast images of cultures on wells treated with Quick-Neuron™ Cholinergic – SeV Complete Kit (objective 20x).

Representative phase contrast images of cultures on glass cover-slips treated with Quick-Neuron™ Cholinergic – SeV Complete Kit.

Protocol/SDS

Protocol

SDS

Others