$349.00

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Skeletal Muscle
Scale: 4 wells of 24-well plate

Description

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Skeletal Muscle
Scale: 4 wells of 24-well plate

Quick-Muscle™ Skeletal allows differentiated skeletal muscle to be rapidly and efficiently produced from human embryonic stem cells and induced pluripotent stem cells within a week. Unlike other products on the market that take nearly a month or longer to generate results, Quick-Muscle™ Skeletal quickly produces skeletal muscle while remaining easy to use, convenient, and efficient. The each option contains enough reagents to use for 4 wells of a 24-well plate.

Features

  • Rapid production: Stop waiting weeks. Quick-Muscle™ Skeletal produces skeletal muscle in within a week.
  • Flexible choice: Complete kit includes all the necessary reagents such as media and well coating.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Muscle™ Skeletal doesn’t.
  • Easy process: No more complex steps! Quick-Muscle™ Skeletal has a simple and easy-to-understand protocol.

Protocol Outline

Applications

Stem Cell Culture, Differentiation and more.

Areas of Interest

Muscular Atrophies, Muscular Growth, Myopathy Diseases, Muscular Disorders, Muscular Dystrophy, Myositis, Neuromuscular Diseases, Sarcopenia

Specifications

Scale 4 wells of a 24-well plate
Contents Three 500 µl tubes and one 2.0 ml tubes
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See product protocol)

Reference

Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko. “Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells.” Development 2016. 143: 3674-3685; doi: 10.1242/dev.139360
Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH (2018). “Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.” Sci Rep. 2018 8(1):1189.

FAQ

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Images


Immuno-staining images of cultures treated by Quick-Muscle™ Skeletal – SeV Complete Kit on Day 5 (20x objective).

Representative phase contrast images of cultures treated with Quick-Muscle™ Skeletal – SeV Complete Kit (10x objective).

MYL gene expression profile of a culture of Quick-Endothelium™ Vascular – mRNA Complete Kit on Day 4

Protocol/SDS

Protocol

SDS

Others