• Quick Pluripotency Testing Kit

$549.00

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Cholinergic Neurons,Skeletal Muscle,Hepatocytes
Scale: 6 wells of 24-well plate

You will get 15% volume discount by buying more than 3 kits!

Description

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Cholinergic Neurons,Skeletal Muscle,Hepatocytes
Scale: 6 wells of 24-well plate

The Quick-Trilineage™ Differentiation Kit allows researchers to assess the pluripotency of their embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) lines in one week. The kit matches the in vivo teratoma formation test to see if all three germ layers can be produced from the ESC/iPSC in question. In this case, neurons, muscle cells, and hepatocytes are rapidly and efficiently produced from human ESCs and iPSCs within one week from a single assay. Unlike other products on the market that take nearly a month or longer to generate results, the Quick-Trilineage™ Differentiation Kit quickly produces differentiated cells while remaining easy to use, convenient, and efficient. The kit contains enough reagents to use for 6 wells (2 wells for each tissue) of a 24-well plate.

Features

  • Rapid production: Stop waiting weeks. The Quick-Trilineage™ Differentiation Kit produces all three germ layers (in the form of neurons, muscle cells, and hepatocytes) in under one week from single assay.
  • All-in-one: Necessary reagents such as media and well coating are all included!
  • No footprint: Other methods leave behind molecular and genetic traces — the Quick-Trilineage™ Differentiation Kit doesn’t.
  • Easy process: No more complex steps! The Quick-Trilineage™ Differentiation Kit has a simple and easy-to-understand protocol.

Protocol Outline

Applications

Pluripotent Stem Cell line establishment, Stem Cell Culture, Differentiation

References

  • Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367
  • Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko. “Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells.” Development 2016. 143: 3674-3685; doi: 10.1242/dev.139360

FAQ

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Specifications

Scale 4 wells of a 24-well plate or 4-well plate
Contents Nine 500 µl tubes, four 15 ml tubes, and two 2.0 ml tubes
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See product protocol)

Images


Gene expression profile of each well of a culture of Quick-Trilineage™ Differentiation Kit on Day 6

Gel electrophoresis of each well of a culture of Quick-Trilineage™ Differentiation Kit on Day 6

Immuno-staining images of each well of a culture of Quick-Trilineage™ Differentiation Kit on Day 6 (20x objective).


Representative images of cultures by Quick-Trilineage™ Differentiation Kit (10x objective).

Protocol/SDS

Protocol

SDS

Others