• Cholinergic neuron

$1,999.00

Elixirgen Scientific’s Cholinergic Neurons are produced with Quick-Neuron™ Cholinergic – SeV Complete Kit and CIRM hPSC repository lines. You can select any lines from the repository to model various diseases and control lines.

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Description

US

Elixirgen Scientific’s Cholinergic Neurons are produced with Quick-Neuron™ Cholinergic – SeV Complete Kit and CIRM hPSC repository lines. You can select any lines from the repository to model various diseases and control lines like follow.

  • 304 Control
  • 65 Alzheimer’s Disease
  • 113 Autism Spectrum Disorder
  • 19 Cerebral Palsy
  • 43 Epilepsy
  • 60 Intellectual Disability
  • And other non-brain disease

Features

  • High efficiency: ~90% TUBB3 positive and ~70% ChAT positive among TUBB3 positive cells.
  • Highly functional: Our neurons show high functionality such as synopsis network bursting with high intensity after few weeks of culturing unlike other iPSC-derived neurons.
  • Consistency: Thanks to fast differentiation, cells show high homogeneity.
  • Flexible choice: You can specify preferred delivery formats (e.g., microtiter plates, MEAs) for live cells in addition to frozen vials.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Cholinergic doesn’t.

Images


Movie depicting the calcium transients of differentiated neurons on Day 7. After loading with Fluo-4, cells were stimulated with 40 Hz pulse.
[Published originally as Supplementary Movie M1 in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]

Repeated multiple action potentials induced by current injections in the current clamp mode (Day 10).
[Published originally as Figure 5g in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]

Immunofluorescence microscopy

Quantitative RT-PCR

Applications

3D Bioprinting, Cell Replacement Therapy, Disease Modeling, Toxicity screening.

Areas of Interest

Diseases Related to the Brain, Spine, and Nerves such as Amyotrophic lateral sclerosis (ALS), multifocal motor neuropathy, primary lateral sclerosis, progressive muscular atrophy, progressive bulbar palsy, or pseudobulbar palsy

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

FAQ

Visit our FAQ page for this product.

Specifications

Form Cryopreserved
Species Human
Donor CIRM hPSC repository. Detail data is available at Coriell Institute individual line webpage
Size 1 vial
# of cells More than 1,000,000 viable cells
Shipping Conditions Dry ice
Storage Conditions Liquid nitrogen

Protocol/SDS

Protocol

SDS