iPSC-derived Mixed Neurons

Human induced pluripotent stem cells (iPSCs) are a powerful tool for studying neuronal activity in vitro. Human iPS cells overcome many of the limitations of other popular models, such as immortalized cell lines and primary mouse neurons, which can be plagued by reproducibility issues and lack of biological relevance. Although human iPS cells have many advantages over existing models, one drawback is that differentiation into the desired cell type is a time and labor intensive process. To address this research bottleneck, Elixirgen Scientific provides a variety of products and services to make research more efficient. Our proprietary technology allows for rapid, reproducible differentiation of human iPSC-derived mixed neurons without sacrificing purity. 

Advantages

  • Fast differentiation and maturation
  • Highly pure population
  • No genetic footprint

Applications

  • Basic research
  • Drug screening
  • Disease modeling

Quick-Neuron™ Mixed Differentiation Kits

Our Quick-Neuron™ Mixed differentiation kits allow researchers to quickly, easily, and efficiently differentiate their iPS cell line into a mixed population of neurons. The kits are available in two scales and are in Sendai virus format to allow for efficient differentiation without creating a genetic footprint.  

Cat no.Product NameDescription
EXGS-QNMSVSeV Complete KitThis kit differentiates human pluripotent stem cells into mixed neurons in 10 days using Sendai Virus.
EXGS-QNMMMaintenance MediumThis medium maintains differentiated mixed neurons.

iPSC-derived Mixed Neurons from Healthy and Affected Donors

Elixirgen Scientific’s human iPSC-derived mixed neurons are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM). All of our iPSC-derived mixed neurons are differentiated using our proprietary technology that utilizes a potent cocktail of key transcription factors to transform iPSCs into high-quality, differentiated mixed neurons. The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like mixed neurons derived from a donor cell line that is not listed in the table below.

Cat no.Donor InformationDisease Status
EXGS-QNMSVF-CW50065-1MHuman iPSC-derived Mixed Neurons (F, 74 yr) Healthy
EXGS-QNMSVF-CW10149-1MHuman iPSC-derived Mixed Neurons (M, 38 yr) Healthy
EXGS-QNMSVF-CW20300-1MHuman iPSC-derived Mixed Neurons (M, 10 yr) Healthy
EXGS-QNMSVF-CW50023-1MHuman iPSC-derived Mixed Neurons (M, 69 yr)Healthy
EXGS-QNMSVF-CW70067-1MHuman iPSC-derived Mixed Neurons (F, 21 yr)Healthy
EXGS-QNMSVF-CW50025-1MHuman iPSC-derived Mixed Neurons (F, 68 yr)Alzheimer’s Disease
EXGS-QNMSVF-CW50113-1MHuman iPSC-derived Mixed Neurons (M, 68 yr)Alzheimer’s Disease
EXGS-QNMSVF-CW50114-1MHuman iPSC-derived Mixed Neurons (F, 72 yr)Alzheimer’s Disease
EXGS-QNMSVF-CW50115-1MHuman iPSC-derived Mixed Neurons (F, 67 yr)Alzheimer’s Disease
EXGS-QNMSVF-CW50137-1MHuman iPSC-derived Mixed Neurons (F, 70 yr)Alzheimer’s Disease
EXGS-QNMSVF-CW50147-1MHuman iPSC-derived Mixed Neurons (M, 71 yr)Alzheimer’s Disease
EXGS-QNMSVF-CW60130-1MHuman iPSC-derived Mixed Neurons (F, 6 yr)Epilepsy
EXGS-QNMSVF-CW60231-1MHuman iPSC-derived Mixed Neurons (M, 6 yr)Epilepsy
EXGS-QNMSVF-CW60236-1MHuman iPSC-derived Mixed Neurons (F, 11 yr) Epilepsy
EXGS-QNMSVF-CW20026-1MHuman iPSC-derived Mixed Neurons (M, 10 yr)Autism Spectrum Disorder
EXGS-QNMSVF-CW20090-1MHuman iPSC-derived Mixed Neurons (F, 18 yr)Autism Spectrum Disorder
EXGS-QNMSVF-CW10130-1MHuman iPSC-derived Mixed Neurons (M, 36 yr)Fatty Liver Disease

Quick-Neuron™ Mixed Differentiation Service

Elixirgen Scientific provides stem cell differentiation services with the world’s fastest turnaround time. Our differentiation method provides homogenous cell cultures without a genetic footprint. Researchers can simply ship live iPSCs to us and receive differentiating or terminally differentiated mixed neurons in as little as one week. Each project is unique, so please contact us with your specifications to receive more information or to receive a quote.

Characterization

hpsc neurons

Mixed neurons display typical neurite outgrowth and expression of neuronal markers. Immunofluorescent staining on Day 10 that demonstrates expression of the pan-neuronal marker TUBB3, the cholinergic neuron marker ChAT, the dopaminergic neuron marker TH, the GABAergic neuron marker PVALB, the glutamatergic neuron marker VGLUT1, and the serotonergic neuron marker TPH2.

Calcium transient event frequency increases with Elixirgen mixed neuron maturation. Neurons loaded with fluorescent calcium indicator Rhod-4 were imaged using the Vala Sciences IC200-Kinetic Image Cytometer® high content imaging system at 4 frames per second for two minutes at 20X magnification. Single-cell analysis using Vala’s CyteSeer® image analysis software captured bursts of increased fluorescence associated with spontaneous firing, implying functional calcium ion channels. Firing frequency increased between Day 14 and Day 21 post-thaw, demonstrating increased functional maturation. n = 50 cells per condition; p = 0.0004. Click here to watch video.