iPSC-derived Excitatory Neurons

Human induced pluripotent stem cells (iPSCs) are a powerful tool for studying neuronal activity in vitro. Human iPS cells overcome many of the limitations of other popular models, such as immortalized cell lines and primary mouse neurons, which can be plagued by reproducibility issues and lack of biological relevance. Although human iPS cells have many advantages over existing models, one drawback is that differentiation into the desired cell type is a time and labor-intensive process. To address this research bottleneck, Elixirgen Scientific provides a variety of products and services to make research more efficient. Our proprietary technology allows for rapid, reproducible differentiation of human iPSC-derived excitatory neurons without sacrificing purity. 

Advantages

  • Fast differentiation and maturation
  • Highly pure population
  • No genetic footprint

Applications

  • Basic research
  • Drug screening
  • Disease modeling

Quick-Neuron™ Excitatory Differentiation Kit & Medium

Our Quick-Neuron™ Excitatory differentiation kit allows researchers to quickly, easily, and efficiently differentiate their iPS cell line into excitatory neurons. 

Cat No.Product NameDescription
EX-SeVQuick-Neuron™ Excitatory - SeV KitThis kit differentiates human pluripotent stem cells into excitatory neurons in 10 days using Sendai Virus.
EX-MMQuick-Neuron™ Excitatory - Maintenance MediumThis medium maintains differentiated excitatory neurons for up to 2 weeks.

iPSC-derived Excitatory Neurons from Healthy and Affected Donors

Elixirgen Scientific’s human iPSC-derived excitatory neurons are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM). All of our iPSC-derived excitatory neurons are differentiated using our proprietary technology that utilizes a potent cocktail of key transcription factors to transform iPSCs into high-quality, differentiated excitatory neurons. The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like excitatory neurons derived from a donor cell line that is not listed in the table below.

Cat No.Product NameDisease Status
EX-SeV-CW50065Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 74 yr)Healthy Control
EX-SeV-CW10149Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 38 yr)Healthy Control
EX-SeV-CW20300Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 10 yr)Healthy Control
EX-SeV-CW50023Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 69 yr)Healthy Control
EX-SeV-CW70067Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 21 yr)Healthy Control
EX-SeV-CW50025Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 68 yr)Alzheimer’s Disease
EX-SeV-CW50113Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 68 yr)Alzheimer’s Disease
EX-SeV-CW50114Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 72 yr)Alzheimer’s Disease
EX-SeV-CW50115Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 67 yr)Alzheimer’s Disease
EX-SeV-CW50137Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 70 yr)Alzheimer’s Disease
EX-SeV-CW50147Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 71 yr)Alzheimer’s Disease
EX-SeV-CW60130Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 6 yr)Epilepsy
EX-SeV-CW60231Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 6 yr)Epilepsy
EX-SeV-CW60236Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 11 yr) Epilepsy
EX-SeV-CW20026Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 10 yr)Autism Spectrum Disorder
EX-SeV-CW20090Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 18 yr)Autism Spectrum Disorder
EX-SeV-CW10130Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 36 yr)Fatty Liver Disease

Quick-Neuron™ Excitatory Differentiation Service

Elixirgen Scientific provides stem cell differentiation services with the world’s fastest turnaround time. Our differentiation method provides homogenous cell cultures without a genetic footprint. Researchers can simply ship live iPSCs to us and receive differentiating or terminally differentiated excitatory neurons in as little as one week. Each project is unique, so please contact us with your specifications to receive more information or to receive a quote.

Characterization

ipsc excitatory neurons

Quick-Neuron™ Excitatory cell cultures express neuronal markers and display typical neurite outgrowth. Immunofluorescent staining that shows expression of the pan-neuronal marker TUBB3 and the glutamatergic neuron-specific marker vGLUT1 on Day 10.

E-PHY

Electrophysiological properties of Quick-Neuron™ Excitatory cell cultures. Excitatory neurons were measured at 5-6 weeks post-thaw using (A) whole-cell path clamp. (B) The neurons showed spontaneous action potentials. (C) Spontaneous post-synaptic current of neuron were observed by voltage clamp measurement at -70mV, suggesting mature synaptic formation between neurons. Data courtesy of E-PHY-SCIENCE.