iPSC-derived Cholinergic Neurons

Human induced pluripotent stem cells (iPSCs) are a powerful tool for studying neuronal activity in vitro. Human iPS cells overcome many of the limitations of other popular models, such as immortalized cell lines and primary mouse neurons, which can be plagued by reproducibility issues and lack of biological relevance. Although human iPS cells have many advantages over existing models, one drawback is that differentiation into the desired cell type is a time and labor intensive process. To address this research bottleneck, Elixirgen Scientific provides a variety of products and services to make research more efficient. Our proprietary technology allows for rapid, reproducible differentiation of human iPSC-derived cholinergic neurons without sacrificing purity. 

Advantages

  • Fast differentiation and maturation
  • Highly pure population
  • No genetic footprint

Applications

  • Basic research
  • Drug screening
  • Disease modeling

Quick-Neuron™ Cholinergic Differentiation Kits & Medium

Our Quick-Neuron™ Cholinergic differentiation kits allow researchers to quickly, easily, and efficiently differentiate their iPS cell line into cholinergic neurons. The kits are available in Sendai virus and synthetic mRNA formats, which give comparable efficiencies and do not leave a genetic footprint.

Cat no.Product NameDescription
EXGS-QNCSVSeV Complete KitThis kit differentiates human pluripotent stem cells into cholinergic neurons in 5 days using Sendai Virus.
EXGS-QNCmRNA Complete KitThis kit differentiates human pluripotent stem cells into cholinergic neurons in 5 days using synthetic mRNA.
EXGS-QNCMMaintenance MediumThis medium maintains differentiated cholinergic neurons.

iPSC-derived Cholinergic Neurons from Healthy and Affected Donors

Elixirgen Scientific’s human iPSC-derived Cholinergic neurons are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM). All of our iPSC-derived Cholinergic neurons are differentiated using our proprietary technology that utilizes a potent cocktail of key transcription factors to transform iPSCs into high-quality, differentiated Cholinergic neurons. The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like Cholinergic neurons derived from a donor cell line that is not listed in the table below.

Cat no.Donor InformationDisease Status
EXGS-QNCSVF-CW50065-1MHuman iPSC-derived Cholinergic Neurons (F, 74 yr) Healthy
EXGS-QNCSVF-CW10149-1MHuman iPSC-derived Cholinergic Neurons (M, 38 yr) Healthy
EXGS-QNCSVF-CW20300-1MHuman iPSC-derived Cholinergic Neurons (M, 10 yr) Healthy
EXGS-QNCSVF-CW50023-1MHuman iPSC-derived Cholinergic Neurons (M, 69 yr)Healthy
EXGS-QNCSVF-CW70067-1MHuman iPSC-derived Cholinergic Neurons (F, 21 yr)Healthy
EXGS-QNCSVF-CW50025-1MHuman iPSC-derived Cholinergic Neurons (F, 68 yr)Alzheimer’s Disease
EXGS-QNCSVF-CW50113-1MHuman iPSC-derived Cholinergic Neurons (M, 68 yr)Alzheimer’s Disease
EXGS-QNCSVF-CW50114-1MHuman iPSC-derived Cholinergic Neurons (F, 72 yr)Alzheimer’s Disease
EXGS-QNCSVF-CW50115-1MHuman iPSC-derived Cholinergic Neurons (F, 67 yr)Alzheimer’s Disease
EXGS-QNCSVF-CW50137-1MHuman iPSC-derived Cholinergic Neurons (F, 70 yr)Alzheimer’s Disease
EXGS-QNCSVF-CW50147-1MHuman iPSC-derived Cholinergic Neurons (M, 71 yr)Alzheimer’s Disease
EXGS-QNCSVF-CW60130-1MHuman iPSC-derived Cholinergic Neurons (F, 6 yr)Epilepsy
EXGS-QNCSVF-CW60231-1MHuman iPSC-derived Cholinergic Neurons (M, 6 yr)Epilepsy
EXGS-QNCSVF-CW60236-1MHuman iPSC-derived Cholinergic Neurons (F, 11 yr) Epilepsy
EXGS-QNCSVF-CW20026-1MHuman iPSC-derived Cholinergic Neurons (M, 10 yr)Autism Spectrum Disorder
EXGS-QNCSVF-CW20090-1MHuman iPSC-derived Cholinergic Neurons (F, 18 yr)Autism Spectrum Disorder
EXGS-QNCSVF-CW10130-1MHuman iPSC-derived Cholinergic Neurons (M, 36 yr)Fatty Liver Disease

Quick-Neuron™ Cholinergic Differentiation Service

Elixirgen Scientific provides stem cell differentiation services with the world’s fastest turnaround time. Our differentiation method provides homogenous cell cultures without a genetic footprint. Researchers can simply ship live iPSCs to us and receive differentiating or terminally differentiated cholinergic neurons in as little as one week. Each project is unique, so please contact us with your specifications to receive more information or to receive a quote.

Characterization

ips-derived cholinergic neurons
Immunofluorescent staining on day 10 that demonstrates typical neurite outgrowth and homogenous expression of the pan-neuronal marker TUBB3 and cholinergic neuron-specific marker ChAT.