Protocol Outline

Images


Immuno-staining images of cultures for Skeletal Muscle derived from CW50065 iPS cell line after 3 days from thawing.


Representative images of cultures of Skeletal Muscle derived from CW50065 iPS cell line after thawing.

Applications

Drug Screening, Toxicity Screening, Precision Medicine, Tissue Chips, 3D-Bioprinting, Transplantation, Research Models, etc.

Areas of Interest

Muscular Atrophies, Muscular Growth, Myopathy Diseases, Muscular Disorders, Muscular Dystrophy, Myositis, Neuromuscular Diseases, Sarcopenia

Reference

Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko. “Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells.” Development 2016. 143: 3674-3685; doi: 10.1242/dev.139360
Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH (2018). “Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.” Sci Rep. 2018 8(1):1189.

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