Protocol Outline

Images


Movie depicting the calcium transients of differentiated neurons on Day 7. After loading with Fluo-4, cells were stimulated with 40 Hz pulse.
[Published originally as Supplementary Movie M1 in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]


Repeated multiple action potentials induced by current injections in the current clamp mode (Day 10 from iPSCs).
[Published originally as Figure 5g in the paper on which this kit is based (Goparaju et al., 2017, Scientific Reports 7, 42367).]


Immuno-staining images of a culture of Cholinergic Neurons derived from CW50065 iPS cell line from 2 days after thawing.


Representative phase contrast images of cultures of Cholinergic Neurons derived from CW50065 iPS cell line after thawing.

Applications

Drug Screening, Toxicity Screening, Precision Medicine, Tissue Chips, 3D-Bioprinting, Transplantation, Research Models, etc.

Areas of Interest

Diseases Related to the Brain, Spine, and Nerves such as Amyotrophic lateral sclerosis (ALS), multifocal motor neuropathy, primary lateral sclerosis, progressive muscular atrophy, progressive bulbar palsy, or pseudobulbar palsy

Reference

Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367

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