Elixirgen Scientific at SLAS
We hope you’ll join us at SLAS 2020 in San Diego, California. Visit us at
Booth #1346 to learn more about how you can use our differentiated human iPS cells and differentiation kits for your high-throughput experiments, such as drug discovery and toxicity screening. All SLAS attendees who stop by our booth will receive an , so don’t miss out! exclusive offer Featured Applications
The high-density multielectrode array (HD-MEA, a) is a powerful tool to analyze activities of a whole neural network as well as single neurons. When our Mixed Neurons were seeded on an HD-MEA plate (MaxTwo, MaxWell Biosystems) with astrocytes, strong spontaneous firing activities were detected as shown in the (b) whole-sample activity map taken after 55 days of in vitro culture. (c) Burst firing showed strong synchronization across the 1,024 active electrodes. These results suggest that the HD-MEA combined with our Mixed Neurons provides an assay system amenable to drug discovery.
Calcium Measurement Assays
Electric stimulation-evoked calcium transients in iPSC derived neurons on Day 7. Fluo-4 loaded cells before electric stimulation in (a) DIC microscopy and (b) fluorescence microscopy. (c) Cells after electric stimulation with 40 Hz pulses for 5 sec. (d) Cells pre-treated with tetrodotoxin (TTX) (0.5 μM) for 2 min to block sodium channels, followed by electric stimulation. (e) Timeline of representative trace of cell, indicated by an arrow in (a). The timings of image capture are indicated as (b–d). (f) Time line trace shows the recovery of cells to evoke calcium transients after TTX wash-off. Citation: Goparaju, S. K. et al. Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. Sci. Rep. 7, 42367; (2017). doi: 10.1038/srep42367
(a) Schematic cross-sectional view of an inkjet cell-printing head (Ricoh). (b) The print head allows multiple types of cells to be placed in a precise manner without cross-contamination. (c) Mixed Neurons at Day 3 post-differentiation were successfully seeded and maintained in 1.5 mm wells with 1.3 μl volume seeded. (d). After 7 days of culturing, Staurosporine was applied and the viability of cells were measured and compared to cells seeded in larger (100 μl) volume wells. The responses were equivalent, which suggests that bioprinting our Mixed Neurons is an effective and efficient method for seeding cells for high-throughput applications such as drug discovery and toxicity screening. Citation: Takagi, D., et al. High-precision 3D inkjet technology for live cell bioprinting. I nt. J. Bioprinting. 5, 2; (2019). doi: 10.18063/ijb.v5i2.208