The Quick-TissueTM Series is a complete reagent set that performs the fastest differentiation of induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs).
For example, Elixirgen Scientific’s Quick-NeuronTM Cholinergic kit performs faster than any of the protocols used by researchers and other companies today. ESCs/iPSCs become functionally mature neurons in only one week. Such speed does not come at the cost of efficiency – past data shows that up to 90% of iPSCs and ESCs differentiate into cholinergic neurons.
The Quick-TissueTM Series employs simple and easy steps to differentiate cells. There are only 4 treatments for the RNA-based kits and only 1 treatment for Sendai Virus vector-based kits.
The Quick-TissueTM Series offers consistent and reproducible cell differentiation. For example, the timing of differentiation, experiment-to-experiment, and well-to-well variation are tightly controlled. These are desirable features, especially for the high throughput screening (HTS) of drugs and for testing the toxicity of chemicals for safety.
The Quick-Tissue Series reagents are composed of cocktails of transcription factors, and thus, they are available in different sizes and scales. Elixirgen Scientific offers small-scale differentiation kits with reagents sufficient to differentiate cells in 4 wells of 24-well microtiter plate. For toxicity screening, Elixirgen Scientific offers a medium size differentiation kit sufficient to differentiate cells in 1x 96-well microtiter plates. For drug screening and other large-scale screening purposes, Elixirgen Scientific offers larger sizes differentiation kits sufficient to differentiate cells in 1000x 96-well microtiter plates.
Thanks to its basis in RNA technology or Sendai Virus technology, the Quick-TissueTM Series treatment does not leave behind a genetic footprint in the cells it differentiates, unlike many common differentiation strategies. This ensures that your experiments with subjects such as disease iPSCs will not be unduly modified during the differentiation process.
Quick-Tissue Series Kits with RNA
The Quick-TissueTM Series kits not marked as “SeV” use advanced RNA technology in order to differentiate your cells without a trace. Since RNA is transcribed in the cytoplasm then used up, there will not be gene expression beyond what is required, and the kit’s RNA will be consumed in order to enact the differentiation.
Quick-Tissue Series Kits with Sendai Virus
The Quick-TissueTM Series SeV kits partially rely on the Sendai Virus (SeV) to cause differentiation for its subject cells. SeV is a RNA-virus – none of the phases of its life cycle involve DNA, so there is little risk of genomic integration. In addition, the particular type of SeV used by Elixirgen Scientific’s kits is temperature sensitive; it is most active at 33 degrees C, then becomes neutralized once its temperature reaches 37 degrees C. This neutralization successfully eliminates the virus from the cells.
With the Quick-Tissue kits, researchers can use cells of their choice and differentiate them into specific cell types. For example, researchers can use human ES cells and human iPS cells from a variety of sources or cells that they have generated by themselves. In addition, a large number of human iPS cells derived from patients with many different kinds of diseases are becoming available from the California Institute for Regenerative Medicine through the Coriell Biorepository. It has also become popular to make specific mutations in human ES and iPS cells by using genome editing technologies such as CRISPR/Cas9. Researchers can use any of these human ES and iPS cells and differentiate them to specific cell types for their own studies.
Elixirgen Scientific provides pluripotent stem cell differentiation services with the world’s fastest turnaround time. Customers can simply ship live iPS/ES cells in a T-25 flask and will receive differentiating or terminally differentiated cells in a week (express service) or two weeks (regular service).
Currently Elixirgen Scientific offers cholinergic neuron, dopaminergic neuron, and skeletal muscle cell differentiation services. More tissue types are coming soon!
Differentiation service price differs based on project requirements, but project design consultation is free.
Do not hesitate to contact us for more details.