What is the Quick-Tissue™ Series?

The Quick-Tissue™ Series is a revolutionary new way to generate human cells, tissues, and organs.

These potent cocktails of transcription factors (TFs) transform your stem cells of choice into high-quality tissues in a scalable, speedy manner.

Efficient delivery of TFs to cells is achieved in two different ways: Synthetic mRNAs or Sendai Virus vectors.

Speedy and Simple

The Quick-Tissue™ Series is a complete reagent set that performs the fastest differentiation of induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs).

For example, Elixirgen Scientific’s Quick-Neuron™ Cholinergic kit performs faster than any of the protocols used by researchers and other companies today. ESCs/iPSCs become functionally mature neurons in only one week. Such speed does not come at the cost of efficiency – past data shows that up to 90% of iPSCs and ESCs differentiate into cholinergic neurons.

The The Quick-Tissue™ Series employs simple and easy steps to differentiate cells. There are only 4 treatments for the RNA-based kits and only 1 treatment for Sendai Virus vector-based kits.

Consistent

The The Quick-Tissue™ Series offers consistent and reproducible cell differentiation. For example, the timing of differentiation, experiment-to-experiment, and well-to-well variation are tightly controlled. These are desirable features, especially for the high throughput screening (HTS) of drugs and for testing the toxicity of chemicals for safety.

Functional

Elixirgen Scientific’s Quick-Neuron™ cells fire strong and unique synchronized bursts when they are measured using multielectrode array (MEA; see below). Robust compound-specific dose responses were observed on MEA using our mixed neuron product. A wide variety of neurons derived from healthy individuals as well as disease patients are available.

Phase contrast image of Mixed Neurons on MEA (day 50 post-thaw)

Multiple subtype-specific markers were examined by immunocytochemistry

Neural network burst data shown here are captured with MED64 Presto (AlphaMED Scientific) 64 days after thawing Mixed Neurons (data provided by Dr. Ikuro Suzuki at Tohoku Institute of Technology).

Use Any Cells

With the Quick-Tissue™ kits, researchers can use cells of their choice and differentiate them into specific cell types. For example, researchers can use human ES cells and human iPS cells from a variety of sources or cells that they have generated by themselves. In addition, a large number of human iPS cells derived from patients with many different kinds of diseases are becoming available from the California Institute for Regenerative Medicine through the Coriell Biorepository. It has also become popular to make specific mutations in human ES and iPS cells by using genome editing technologies such as CRISPR/Cas9. Researchers can use any of these human ES and iPS cells and differentiate them to specific cell types for their own studies.

Diseases on your choice

The California Institute for Regenerative Medicine (CIRM) has granted a license to Elixirgen Scientific to commercially distribute differentiated tissues derived from CIRM’s patient iPS cell lines. If you are interested in neurons derived from particular disease-associated or multiple control iPS cell lines, please contact us.

The CIRM iPSC repository includes over 1,500 iPSC lines, encompasses 5 different disease classes, and contains age-matched controls for each disease patient iPSC line. Further details such as demographic and clinical data about the collection can be found at here.

Cholinergic neurons from CIRM Alzheimer’s disease line, CW50114

Category No. of iPSC lines
Control Cell Lines 302
Brain Diseases 304
Alzheimer's Disease 65
Autism Spectrum Disorder 113
Cerebral Palsy 19
Epilepsy 47
Intellectual Disability 60
Blinding Eye Diseases 175
Age-related Macular Degeneration 120
Diabetic Retinopathy 34
Glaucoma, Primary Open Angle 21
Heart Diseases 442
Cardiomyopathy, Dilated 313
Cardiomyopathy, Hypertrophic 74
Cardiomyopathy, Left Ventricular Non-Compaction 9
Cardiomyopathy, Arrhythmogenic Right Ventricular 2
Cardiomyopathy, Restrictive 1
Cardiomyopathy, Other 43
Liver Diseases 131
Hepatitis C 93
NASH and Steatosis 38
Lung diseases 191
Idiopathic Pulmonary Fibrosis 191

Genome-corrected iPS cells

Differentiated skeletal muscle cells from genome-corrected iPS cells (B,D) show loss of disease specific RNA foci compared to cells from non-corrected iPS cells (A, C).

Wang et al., (2018). Therapeutic Genome Editing for Myotonic Dystrophy Type 1 Using CRISPR/Cas9. Molecular Therapy 2018;26:2617. [PubMed] [Molecular Therapy] [Kindly provided by Dr. Xia, University of New Mexico]

Scalable

The The Quick-Tissue™ Series reagents are composed of cocktails of transcription factors, and thus, they are available in different sizes and scales. Elixirgen Scientific offers small-scale differentiation kits with reagents sufficient to differentiate cells in 4 wells of 24-well microtiter plate. For toxicity screening, Elixirgen Scientific offers a medium size differentiation kit sufficient to differentiate cells in 1x 96-well microtiter plates. For drug screening and other large-scale screening purposes, Elixirgen Scientific offers larger sizes differentiation kits sufficient to differentiate cells in 100x 96-well microtiter plates.

No Footprint

Thanks to its basis in RNA technology or Sendai Virus technology, the The Quick-Tissue™ Series treatment does not leave behind a genetic footprint in the cells it differentiates, unlike many common differentiation strategies. This ensures that your experiments with subjects such as disease iPSCs will not be unduly modified during the differentiation process.

Quick-Tissue™ Series Kits with RNA

The The Quick-Tissue™ Series kits not marked as “SeV” use advanced RNA technology in order to differentiate your cells without a trace. Since RNA is transcribed in the cytoplasm then used up, there will not be gene expression beyond what is required, and the kit’s RNA will be consumed in order to enact the differentiation.

Quick-Tissue™ Series Kits with Sendai Virus

The The Quick-Tissue™ Series SeV kits partially rely on the Sendai Virus (SeV) to cause differentiation for its subject cells. SeV is a RNA-virus – none of the phases of its life cycle involve DNA, so there is little risk of genomic integration. In addition, the particular type of SeV used by Elixirgen Scientific’s kits is temperature sensitive; it is most active at 33 degrees C, then becomes neutralized once its temperature reaches 37 degrees C. This neutralization successfully eliminates the virus from the cells.

Product Pipeline

portofolio
Tissue / Cell TypeProductiPSC-derived cellsReagent KitDifferentiation ServicesAdditional Info

Mixed Neuron

Quick-Neuron™ Mixed

This kit allows users to differentiate human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), into tubulin β III (TUBB3)-positive neurons within 10 days. These neurons are committed to becoming mixed neurons, which are choline acetyltransferase (ChAT, a cholinergic neuron marker), tyrosine hydroxylase (TH, a dopaminergic neuron marker), parvalbumin (PVALB, a GABAergic neuron marker), vesicular glutamate transporter 1 (vGLUT1, a glutamatergic neuron marker), and tryptophan hydroxylase 2 (TPH2, a serotonergic neuron marker)-positive.

Cholinergic Neuron

Quick-Neuron™ Cholinergic

This kit allows users to differentiate human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), into tubulin β III (TUBB3)-positive neurons within one week. These neurons are committed to becoming cholinergic neurons.

Dopaminergic Neuron

Quick-Neuron™ Dopaminergic

This kit allows users to differentiate human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), into tubulin β III (TUBB3)-positive neurons within 10 days. These neurons are committed to becoming dopaminergic neurons.

GABAergic Neuron

Quick-Neuron™ GABAergic

This kit allows users to differentiate human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), into tubulin β III (TUBB3)-positive neurons within 10 days. These neurons are committed to becoming GABAergic neurons.

Skeletal Muscle

Quick-Muscle™ Skeletal

This kit allows users to differentiate human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), into myosin heavy chain (MHC)-positive muscle cells within one week.

Hepatocyte

Quick-Hepatocyte™

This kit allows users to differentiate human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), into albumin-positive hepatocyte-like cells within 9 days.

Vascular Endothelial Cells

Quick-Endothelium™ Vascular

Coming Soon!

3D Brain Organoids

Quick-miniBrain™

Coming Soon!

CD45+ Cells

Quick-CD45+ Cells

Coming Soon!

Fields of Impact

Applications of Quick-Tissue™ Technologies

Drug Screening

Pharmaceutical companies and researchers can use the Quick-Tissue™ Series’ kits for drug screening. Our kits are best suited to the high throughput screening (HTS) with robots and robotic platforms.
Read More >>

Toxicity Screening

Differentiated cells produced by the Quick-Tissue™ Series kits could be used quickly and effectively for toxicity screening of industrial and environmental substances.
Read More >>

Precision Medicine

“Precision Medicine” can help select the most suitable drugs for individual patients (“personalized or tailored medicine”). Our kits enable these goals through differentiating patient-specific iPS cells.
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Tissue Chips

“Tissue Chips or organs-on-chips” can eventually be used for testing safety and efficacy of drug candidates before any testing in humans. Our kits can significantly accelerate these processes.
Read More >>

3-D Bioprinting

The Quick-Tissue™ Series’ kits can be used to differentiate stem cells on top of 3-D printed scaffolds – currently one of the most common methods for such printing.
Read More >>

Transplantation

One key paradigm in Regenerative Medicine is to differentiate human ES and iPS cells into desired cell types and transplant them to patients in need of these cells/tissue/organs.
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Research Models

Researchers have traditionally used non-human cells for basic and applied biomedical research. The Quick-Tissue™ series increases the availability of human differentiated cells in biomedical research.
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Lower Barrier of Entry

The simplicity and cost of the Quick-Tissue™ Series ensures that even novice scientists can conduct advanced stem cell research.
Read More >>

Services

Cell Differentiation Services

Cell Differentiation Service overview

Stop waiting months for tissues!

Elixirgen Scientific provides pluripotent stem cell differentiation services with the world’s fastest turnaround time. Customers can simply ship live iPS/ES cells in a T-25 flask and will receive differentiating or terminally differentiated cells in a week (express service) or two weeks (regular service).

Currently Elixirgen Scientific offers cholinergic neuron, dopaminergic neuron, and skeletal muscle cell differentiation services. More tissue types are coming soon!

Differentiation service price differs based on project requirements, but project design consultation is free.

Do not hesitate to contact us for more details.

Service Prices