The Quick-Tissue™ Series is a revolutionary new way to generate human cells, tissues, and organoids from stem cells.
Elixirgen Scientific’s proprietary transcription factor-based technology allows for rapid, reproducible differentiation of stem cells (iPS or ES) into the desired cell and tissue types without sacrificing purity. Our technology is available in three formats to best fit the needs of individual researchers.
– Stem cell differentiation kits
– Human iPSC-derived cells
– Stem cell differentiation services
Speedy and Simple
The Quick-Tissue™ Series is a complete reagent set that performs the fastest differentiation of induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs).
For example, Elixirgen Scientific’s Quick-Neuron™ Cholinergic kit performs faster than any of the protocols used by researchers and other companies today. ESCs/iPSCs become functionally mature neurons in only one week. Such speed does not come at the cost of efficiency – past data shows that up to 90% of iPSCs and ESCs differentiate into cholinergic neurons.
Elixirgen Scientific’s Quick-Neuron™ cells fire strong and unique synchronized bursts when they are measured using multielectrode array (MEA; see below). Robust compound-specific dose responses were observed on MEA using our mixed neuron product. A wide variety of neurons derived from healthy individuals as well as disease patients are available.
Phase contrast image of Mixed Neurons on MEA (day 50 post-thaw)
Multiple subtype-specific markers were examined by immunocytochemistry
Use Any Cells
With the Quick-Tissue™ kits, researchers can use cells of their choice and differentiate them into specific cell types. For example, researchers can use human ES cells and human iPS cells from a variety of sources or cells that they have generated by themselves. In addition, a large number of human iPS cells derived from patients with many different kinds of diseases are becoming available from the California Institute for Regenerative Medicine through the Coriell Biorepository. It has also become popular to make specific mutations in human ES and iPS cells by using genome editing technologies such as CRISPR/Cas9. Researchers can use any of these human ES and iPS cells and differentiate them to specific cell types for their own studies.
Diseases of your choice
The California Institute for Regenerative Medicine (CIRM) has granted a license to Elixirgen Scientific to commercially distribute differentiated tissues derived from CIRM’s patient iPS cell lines. If you are interested in neurons derived from particular disease-associated or multiple control iPS cell lines, please contact us.
The CIRM iPSC repository includes over 1,500 iPSC lines, encompasses 5 different disease classes, and contains age-matched controls for each disease patient iPSC line. Further details such as demographic and clinical data about the collection can be found at here.
|Category||No. of iPSC lines|
|Control Cell Lines||302|
|Autism Spectrum Disorder||113|
|Blinding Eye Diseases||175|
|Age-related Macular Degeneration||120|
|Glaucoma, Primary Open Angle||21|
|Cardiomyopathy, Left Ventricular Non-Compaction||9|
|Cardiomyopathy, Arrhythmogenic Right Ventricular||2|
|NASH and Steatosis||38|
|Idiopathic Pulmonary Fibrosis||191|
Genome-corrected iPS cells
Differentiated skeletal muscle cells from genome-corrected iPS cells (B,D) show loss of disease specific RNA foci compared to cells from non-corrected iPS cells (A, C).
Wang et al., (2018). Therapeutic Genome Editing for Myotonic Dystrophy Type 1 Using CRISPR/Cas9. Molecular Therapy 2018;26:2617. [PubMed] [Molecular Therapy] [Kindly provided by Dr. Xia, University of New Mexico]
The The Quick-Tissue™ Series reagents are composed of cocktails of transcription factors, and thus, they are available in different sizes and scales. Elixirgen Scientific offers small-scale differentiation kits with reagents sufficient to differentiate cells in 4 wells of 24-well microtiter plate. For toxicity screening, Elixirgen Scientific offers a medium size differentiation kit sufficient to differentiate cells in 1x 96-well microtiter plates. For drug screening and other large-scale screening purposes, Elixirgen Scientific offers larger sizes differentiation kits sufficient to differentiate cells in 100x 96-well microtiter plates.
Thanks to its basis in RNA technology or Sendai Virus technology, the The Quick-Tissue™ Series treatment does not leave behind a genetic footprint in the cells it differentiates, unlike many common differentiation strategies. This ensures that your experiments with subjects such as disease iPSCs will not be unduly modified during the differentiation process.
Quick-Tissue™ Series Kits with RNA
The The Quick-Tissue™ Series kits not marked as “SeV” use advanced RNA technology in order to differentiate your cells without a trace. Since RNA is transcribed in the cytoplasm then used up, there will not be gene expression beyond what is required, and the kit’s RNA will be consumed in order to enact the differentiation.
Quick-Tissue™ Series Kits with Sendai Virus
The The Quick-Tissue™ Series SeV kits partially rely on the Sendai Virus (SeV) to cause differentiation for its subject cells. SeV is a RNA-virus – none of the phases of its life cycle involve DNA, so there is little risk of genomic integration. In addition, the particular type of SeV used by Elixirgen Scientific’s kits is temperature sensitive; it is most active at 33 degrees C, then becomes neutralized once its temperature reaches 37 degrees C. This neutralization successfully eliminates the virus from the cells.
Stop waiting months for tissues. Elixirgen Scientific provides stem cell differentiation services with the world’s fastest turnaround time. Simply send us your human pluripotent stem cells (iPS or ES cells) and receive differentiating or terminally differentiated cells in just 1-2 weeks. Our proprietary transcription factor-based stem cell differentiation services produce highly pure populations without a genetic footprint. All of our hPSC differentiation services begin with a free consultation with one of our experts so that we can tailor our services to best fit your needs.
|Tissue / Cell Type||Product||Differentiation Kit||Human iPSC-derived Cells||Differentiation Services|
Vascular Endothelial Cells
3D Brain Organoids
Pharmaceutical companies and researchers can use the Quick-Tissue™ Series’ kits for drug screening. Our kits are best suited to the high throughput screening (HTS) with robots and robotic platforms.
Differentiated cells produced by the Quick-Tissue™ Series kits could be used quickly and effectively for toxicity screening of industrial and environmental substances.
The Quick-Tissue™ Series’ kits can be used to differentiate stem cells on top of 3-D printed scaffolds – currently one of the most common methods for such printing.
“Tissue Chips or organs-on-chips” can eventually be used for testing safety and efficacy of drug candidates before any testing in humans. Our kits can significantly accelerate these processes.